Paste here your genomic sequence that you want watermarks generated for. Only A, T, G, and C are acceptable but can be either capital or lowercase. Spaces will be removed. Ns are not allowed currently.

While originally thought to be universal, the codon translation table can vary in some species. Select the species that your watermarks are being generated in. If your species uses the general codon translation table, select 'Standard Codon Table' if it is not otherwise listed.

This is the minimum melting temperature that your watermarks will be required to be estimated at.

This is the maximum melting temperature that your watermarks will be required to be estimated at.

This value should be the minimum percentage of nucleotides within your watermark sequence that you want changed to different nucleotides than what is found in the reference genome. Ultimately having a different enough variation is your validation that your process worked (i.e. you 'see' your watermarks).

This is the smallest number of nucleotides (including watermarks) that should make up your amplification region. This is the region that will be amplified by PCR in order to validate that your synthetic gene is present.

This is the largets number of nucleotides (including watermarks) that should make up your amplification region. This is the region that will be amplified by PCR in order to validate that your synthetic gene is present.